< BK21 플러스 BEST 정보기술 사업단 세미나 개최 안내 >

개최일시 : 2016년 04월 27일 수요일 15:00 ~ 17:00

개최장소 : 제1공학관 A320호

세미나 제목 : Seeing the Unseen with Single-Molecule Perspective

발표초록 :

Handling molecules one at a time provides a unique opportunity to address fundamental biological problems. In this seminar, I will talk about our two recent experiments that cannot be paralleled in conventional bulk measurements. First, by combining two orthogonal techniques of single-molecule FRET and magnetic tweezers, we reveal how the 20S particle consisting of NSF and -SNAP disassembles single SNARE complexes [1-4]. We observed that NSF exploits only one round of ATP binding and hydrolysis to disassemble the SNARE complex, one of the most stable protein complexes, which suggests an incredibly high mechanical efficiency of NSF compared to other AAA+ ATPases. Second, by employing real-time single-molecule fluorescence imaging as a detection scheme, we have recently demonstrated a single-molecule version of the co-immunoprecipitation (co-IP) analysis, which provides an improvement of five orders of magnitude in the time resolution [5,6]. With the single-molecule sensitivity and millisecond time resolution, it is possible to detect changes in the protein-protein interactions in a given tumour tissue. This will shed light onto the molecular lesions that drive individual cancers at the level of protein-protein interactions. I will close the talk with brief outlook for future researches.

강연자 : 윤태영 교수 / 연세대학교 Yonsei-IBS Institute

초청자 : 전기전자공학과 교수 김동현A